Download Alkaliphiles: Genetic Properties and Applications of Enzymes by Koki Horikoshi PDF

By Koki Horikoshi

The pioneering paintings of Dr. Koki Horikoshi at the discovery, learn and functions of alkaliphilic micro organism are defined right here of their entirety. The isolation, distribution and taxonomy of alkaliphilic microorganisms in addition to their telephone constitution and body structure are mentioned for a uncomplicated realizing of those entities. The molecular biology and genome sequencing of a few alkaliphilic bacterial lines also are presented.

Part II of the quantity specializes in enzymes of alkaliphiles and their functions. those contain alkaline proteases, starch-degrading enzymes and various others. a few of these enzymes are presently in huge advertisement use as laundry detergent ingredients and in wastewater treatment.

Alkaliphiles are a comparatively fresh box of analysis that may without doubt offer avenues to quite a lot of additional discoveries and functions for a brand new new release of employees in bioscience and technology.

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Extra resources for Alkaliphiles: Genetic Properties and Applications of Enzymes

Example text

5 Strain A-40-2 2b-2 C-11 C-125 Y-25 A-59 C-3 C-59-2 M-29 57-1 B. 82 Molar ratio to meso-iyA? Abbreviations: Ala, alanine ; GlcN, lucosamine ; Glu, glutamic acid ; m^50-DAP, m^5o-diaminopimelic acid ; Mur, muramic acid. Essentially, the composition of peptidoglycan was not changed whether the strain was cultured at pH 7 or 10. It was therefore concluded that all of the peptidoglycans of the alkaliphilic Bacillus strains so far examined are of the Aly-type of peptidoglycan, which is found in the majority of the strains of the genus Bacillus.

1% glucose at various pH values. Measurement of fluorescence intensity' of the intracellular BCECF is detailed in the text. Cytoplasmic pH of the cells was calculated mainly from the Ratioslo 450- The pH above 9 was calculated from /sio. (Reproduced with permission from R. 1 Growth Conditions 41 in response to the culture pH. 8. 5, the mutant exhibited poor growth rates, only 20% that of parental C-125. The mutant C-125-9 restores pig synthesis by the introduction of the gene ''tupA' cloned from the parental strain and its alkaliphily.

Kudo et a l . 6 kb of common sequence between pALKl and pALK2 (Fig. 8). Plasmid pALKl conferred alkaliphily on mutant 18224 but not on mutant 31154. 5) but not after the introduction of pALKl. These results indicate that there are at least two factors involved in alkaliphily located in a closed linked region of chromosomal DNA, although the precise functions of these two DNA fragments are not known yet. These results strongly suggest that at least two genetic loci that are required for alkaliphily and that they are clustered on the chromosome.

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