Download A Practical Guide to Assay Development and High-Throughput by Taosheng Chen PDF

By Taosheng Chen

The advance of appropriate assays, the combination of acceptable expertise, and the powerful administration of the fundamental infrastructure are all severe to the luck of any high-throughput screening (HTS) exercise. in spite of the fact that, few scientists have the multidisciplinary event had to keep an eye on all features of an HTS drug discovery undertaking. a pragmatic consultant to Assay improvement and High-Throughput Screening in Drug Discovery integrates the adventure of various specialists who supply basic and useful counsel throughout a variety of occasions. The ebook first discusses assay advancements for vital goal sessions similar to protein kinases and phosphatases, proteases, nuclear receptors, G protein-coupled receptors, ion channels, and warmth surprise proteins. It subsequent examines assay advancements for mobilephone viability, apoptosis, and infectious illnesses. The members discover the applying of rising applied sciences and structures, together with image-based excessive content material screening, RNA interference, and first cells. eventually, they speak about the fundamental elements of the built-in HTS approach, akin to screening automation, compound library administration, the screening of ordinary items from botanical resources, and screening informatics. Designed to encourage researchers to carry extra advances to the sector, this quantity offers useful assistance on tips to begin, validate, optimize, and deal with a bioassay meant to monitor huge collections of compounds. Drawing at the wisdom from specialists actively enthusiastic about assay improvement and HTS, it is a source that's either accomplished and targeted.

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Additional info for A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery (Critical Reviews in Combinatorial Chemistry)

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Thus, for making suitable conclusions for assay design, knowledge of the kinetic and binding constants of receptors and enzymes, such as Kd, kcat, Km, Bmax, is useful. Stoichiometric information, such as the number of enzyme molecules per assay, may also be useful because it can serve as a guideline to ensure that the assays are maximally sensitive to the mechanism of action one wants to discover. Problems in assay development often occur when the conditions required for sensitivity to the desired mechanism of action do not yield the best conditions for statistical reproducibility; therefore, compromises and balances of these two opposing factors must be often made.

Et al. 2007. Three mechanistically distinct kinase assays compared: measurement of intrinsic ATPase activity identified the most comprehensive set of ITK inhibitors. J. Biomol. Screen. 12, 70–83. Koresawa, M. and T. Okabe. 2004. High-throughput screening with quantitation of ATP consumption: a universal non-radioisotope, homogeneous assay for protein kinase. Assay Drug Dev. Technol. 2, 153–160. R. 2006. Principles of Fluorescence Spectroscopy. 3rd ed. Berlin: Springer. S. and Q. Wang. 2007. Seeing is believing: peptide-based fluorescent sensors of protein tyrosine kinase activity.

2005) and McCain and Zhang (2001) for tyrosine phosphatases are available. It is often necessary to provide additives such as protease inhibitors to prevent digestion of the assay components or phosphatase inhibitors such as vanadate to keep the product of a kinase assay intact and protected from contaminating phosphatases. In addition, it is often necessary to quench assays or to add a stopping buffer such as EDTA for kinases or vanadate for phosphatases at the end of the reaction and before reading in an MTP plate reader.

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